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Tubulin Beta 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99382

Novus Biologicals, part of Bio-Techne
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NBP2-99382-100ul
NBP2-99382-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Tubulin Beta 4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Tubulin Beta 4 Antibody

Western Blot: Tubulin Beta 4 Antibody [NBP2-99382]

Western Blot: Tubulin Beta 4 Antibody [NBP2-99382]

Western Blot: Tubulin Beta 4 Antibody [NBP2-99382] - Anti-TUBB4A rabbit polyclonal antibody at 1:5000 dilution.
Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP2-99382]

Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody [NBP2-99382]

Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP2-99382] - Immunofluorescence staining of Tubulin Beta 4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Tubulin Beta 4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoskeleton.

Applications for Tubulin Beta 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:2000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tubulin Beta 4

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Long Name

Tubulin beta-4A chain

Alternate Names

TUBB4, TUBB4A, Tubulin 5 beta

Gene Symbol

TUBB4A

Additional Tubulin Beta 4 Products

Product Documents for Tubulin Beta 4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tubulin Beta 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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