TUFM Antibody (1A2)
Novus Biologicals, part of Bio-Techne | Catalog # H00007284-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TUFM (AAH01633, 40 a.a. ~ 455 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAATTKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG
Specificity
TUFM - Tu translation elongation factor, mitochondrial (1A2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for TUFM Antibody (1A2)
![Western Blot: TUFM Antibody (1A2) [H00007284-M03]](https://resources.bio-techne.com/images/products/TUFM-Antibody-1A2-Western-Blot-H00007284-M03-img0004.jpg "Western Blot: TUFM Antibody (1A2) [H00007284-M03]")
Western Blot: TUFM Antibody (1A2) [H00007284-M03]
Western Blot: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM expression in transfected 293T cell line by TUFM monoclonal antibody (M03), clone 1A2. Lane 1: TUFM transfected lysatE (49.9 KDa). Lane 2: Non-transfected lysate.![Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03]](https://resources.bio-techne.com/images/products/TUFM-Antibody-1A2-Immunoprecipitation-H00007284-M03-img0002.jpg "Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03]")
Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03]
Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM transfected lysate using anti-TUFM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TUFM rabbit polyclonal antibody.
Sandwich ELISA: TUFM Antibody (1A2) [H00007284-M03] - Detection limit for recombinant GST tagged TUFM is 0.3 ng/ml as a capture antibody.
Applications for TUFM Antibody (1A2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TUFM
Alternate Names
EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial
Entrez Gene IDs
7284 (Human)
Gene Symbol
TUFM
UniProt
Additional TUFM Products
Product Documents for TUFM Antibody (1A2)
Product Specific Notices for TUFM Antibody (1A2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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