TUFM Antibody (CL2242)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36751
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Predicted:
Mouse (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot
Cited:
Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2242
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSI
Epitope
RDLEKPFLLPVEAVY
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reactivity reported in scientific literature (PMID:31801089).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TUFM Antibody (CL2242)
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]
Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in MCF7 cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]
Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.Applications for TUFM Antibody (CL2242)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunoprecipitation reported in scientific literature (PMID:31801089).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TUFM
Alternate Names
EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial
Gene Symbol
TUFM
UniProt
Additional TUFM Products
Product Documents for TUFM Antibody (CL2242)
Product Specific Notices for TUFM Antibody (CL2242)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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