TUFM Antibody (CL2245) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44457
![TUFM Antibody (CL2245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202512163643.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-28220251219542.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2245
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P49411, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TUFM Antibody (CL2245) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]
Staining in MCF7 cell line with Anti-TUFM monoclonal antibody) .![TUFM Antibody (CL2245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-28220251214536.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]
Staining in U251 cell line with Anti-TUFM monoclonal antibody) .![TUFM Antibody (CL2245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202512145356.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]")
Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]
Staining in HeLa cell line with Anti-TUFM monoclonal antibody) .![TUFM Antibody (CL2245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202512163652.jpg "Immunocytochemistry/ Immunofluorescence: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Western Blot: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-28220251273768.jpg "Western Blot: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202512303827.jpg "Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202513112826.jpg "Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202513112876.jpg "Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]")
![TUFM Antibody (CL2245) - Azide and BSA Free Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]](https://resources.bio-techne.com/images/products/nbp3-44457_mouse-tufm-mab-cl2245-azide-and-bsa-free-282202513401755.jpg "Immunohistochemistry-Paraffin: TUFM Antibody [NBP3-44457]")
Applications for TUFM Antibody (CL2245) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized in 5% Trehalose.
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TUFM
Alternate Names
EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial
Gene Symbol
TUFM
Additional TUFM Products
Product Documents for TUFM Antibody (CL2245) - Azide and BSA Free
Product Specific Notices for TUFM Antibody (CL2245) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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