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TUFM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32051

Novus Biologicals, part of Bio-Techne
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NBP1-32051

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for TUFM Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TUFM Antibody

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-32051]

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-32051]

Immunohistochemistry-Paraffin: TUFM Antibody [NBP1-32051] - Paraffin-embedded SNU16 xenograft, using antibody at 1:100 dilution.
Western Blot: TUFM Antibody [NBP1-32051]

Western Blot: TUFM Antibody [NBP1-32051]

Western Blot: TUFM Antibody [NBP1-32051] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:2000.
TUFM Antibody

Immunoprecipitation: TUFM Antibody [NBP1-32051] -

Immunoprecipitation: TUFM Antibody [NBP1-32051] - TUFM antibody [N3C3] immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 ug HepG2 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 ug TUFM antibody [N3C3] (NBP1-32051)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody [N3C3] (NBP1-32051) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for TUFM Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TUFM

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

Additional TUFM Products

Product Documents for TUFM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TUFM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.