TWA1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32596
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Predicted:
Bovine (97%), Chicken (97%), Mouse (99%), Rhesus Macaque (100%), Xenopus (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TWA1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (96%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TWA1 Antibody
Western Blot: TWA1 Antibody [NBP1-32596]
Western Blot: TWA1 Antibody [NBP1-32596] - ARMC8 HEK293 CRISPR knockout cells were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-46279-5), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: TWA1 Antibody [NBP1-32596]
Immunocytochemistry/Immunofluorescence: TWA1 Antibody [NBP1-32596] - Paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596]
Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.Applications for TWA1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TWA1
Alternate Names
bA305P22.1, C20orf11, chromosome 20 open reading frame 11, FLJ20602, GID complex subunit 8 homolog (S. cerevisiae), Twa1, TWA1protein C20orf11, two hybrid associated protein 1, Two hybrid-associated protein 1 with RanBPM
Gene Symbol
GID8
Additional TWA1 Products
Product Documents for TWA1 Antibody
Product Specific Notices for TWA1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.