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TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09099

Novus Biologicals, part of Bio-Techne
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NBP3-09099

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Functional, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # ITEM-4

Format

Low Endotoxin, Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

human TWEAK R/TNFRSF12-transfected P815 cells

Specificity

The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099]

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099]

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Separation of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099]

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099]

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Surface staining pattern of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

Applications

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 0.2 um filter sterilized

Format

Low Endotoxin, Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TWEAK R/TNFRSF12

TWEAK (otherwise known as TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A), is a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Alternate Names

CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR

Gene Symbol

TNFRSF12A

Additional TWEAK R/TNFRSF12 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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