TWEAK/TNFSF12 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76695
Key Product Details
Validated by
Tagged Protein Expression
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against recombinant human TWEAK/TNFSF12 protein.
Specificity
Human TWEAK/TNFSF12 has 2 isoforms, including isoform 1 (249aa, 27kD) and isoform 2 (330aa, 36kD). Mouse TWEAK/TNFSF12 has one isoform (249aa, 27kD) and Rat TWEAK/TNFSF12 also has one isoform (249aa, 27kD).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27, 36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TWEAK/TNFSF12 Antibody - BSA Free
Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]
Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]
Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.Western Blot: TWEAK/TNFSF12 AntibodyBSA Free [NBP1-76695]
Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.Applications for TWEAK/TNFSF12 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
5-10 ug/mL
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
0.5-4 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TWEAK/TNFSF12
Long Name
TNF-like and Weak Inducer of Apoptosis
Alternate Names
TNFSF12
Entrez Gene IDs
8742 (Human)
Gene Symbol
TNFSF12
UniProt
Additional TWEAK/TNFSF12 Products
Product Documents for TWEAK/TNFSF12 Antibody - BSA Free
Product Specific Notices for TWEAK/TNFSF12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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