Twist-1 Antibody (3E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00007291-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat, Canine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Twist-1 Antibodies »

Product Specifications

Immunogen

TWIST1 (NP_000465, 100 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH

Specificity

TWIST1 - twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Twist-1 Antibody (3E11)

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01] - TWIST1 monoclonal antibody (M01), clone 3E11. Analysis of TWIST1 expression in rat testis.
Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01] - Western Blot analysis in rat brain.
Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01]

Western Blot: Twist-1 Antibody (3E11) [H00007291-M01] - Analysis of TWIST1 over-expressed 293 cell line, cotransfected with TWIST1 Validated Chimera RNAi ( Cat # H00007291-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TWIST1 monoclonal antibody (M01), clone 3E11 (Cat # H00007291-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for Twist-1 Antibody (3E11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. ICC/IF usage reported in scientific literature (PMID: 25002532).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Twist-1

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Long Name

Twist Related Protein 1 [Drosophila]

Alternate Names

ACS3, BPES3, H-twist, Twist1

Entrez Gene IDs

7291 (Human)

Gene Symbol

TWIST1

OMIM

101 (Human)

UniProt

NP_000465 (Human)

Additional Twist-1 Products

Product Documents for Twist-1 Antibody (3E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Twist-1 Antibody (3E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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