Twist-2 Antibody (3C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00117581-M01

Novus Biologicals, part of Bio-Techne
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H00117581-M01
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

CyTOF-ready, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Twist-2 Antibodies »

Product Specifications

Immunogen

TWIST2 (AAH33168, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGSWSMSASH

Specificity

TWIST2 - twist homolog 2 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Twist-2 Antibody (3C8)

Western Blot: Twist-2 Antibody (3C8) [H00117581-M01]

Western Blot: Twist-2 Antibody (3C8) [H00117581-M01]

Western Blot: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M01), clone 3C8.Lane 1: TWIST2 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01]

Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01]

Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of monoclonal antibody to TWIST2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01]

Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01]

Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Immunoperoxidase of monoclonal antibody to TWIST2 ( Cat # H00117581-M01 ) on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml]
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Applications for Twist-2 Antibody (3C8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Twist-2

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype.

Long Name

Twist Related Protein 2 [Drosophila]

Alternate Names

bHLHa39, DERMO1, Twist2

Entrez Gene IDs

117581 (Human)

Gene Symbol

TWIST2

OMIM

607556 (Human)

UniProt

AAH33168 (Human)

Additional Twist-2 Products

Product Documents for Twist-2 Antibody (3C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Twist-2 Antibody (3C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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