CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Proximity Ligation Assay, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Knockout Validated, Proximity Ligation Assay, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # JY2

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TXNIP Antibodies »

Product Specifications

Immunogen

Human recombinant TXNIP

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34576095). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for TXNIP Antibody (JY2) - Azide and BSA Free

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - Analysis of TXNIP (NBP1-54578), ACTB, CASP1 (cleaved), and IL1B (cleaved) protein in livers from mice treated with WY-14643 for 48 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33203882/) licensed under a CC-BY license.

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - An intracellular stain was performed on THP-1 cells with TXNIP Antibody (JY2) NBP1-54578AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per lane. Image from verified customer review.

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population and the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean SEM of 3 samples per group.0.05,0.01,0.005, and0.001. Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway. J Diabetes Res (2019)

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules and glomeruli.

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules with negligible staining in glomeruli.

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) [NBP1-54578] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] -

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population & the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 beta ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 beta ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean ± SEM of 3 samples per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, & ∗∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31886288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

View 8 more images

Applications for TXNIP Antibody (JY2) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

2-5 ug/0.1x10^6 cells

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2ug/200ul of cell extract from 5x10^6 cells

Knockout Validated

reported in scientific literature (Brocker et al)

Western Blot

1 ug/ml

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-54578 in the following applications:

Immunohistochemistry-Frozen (1 Review)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TXNIP

Vitamin D3 up-regulated protein 1 (VDUP-1), also called Thioredoxin (TRX)-interacting protein, is an endogenous inhibitor of TRX. Redox-dependent regulation of VDUP-1 by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of VDUP-1 to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that TXNIP plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.

Alternate Names

HHCPA78, THIF, thioredoxin binding protein 2, thioredoxin interacting protein, Thioredoxin-binding protein 2, thioredoxin-interacting protein, upregulated by 1,25-dihydroxyvitamin D-3, VDUP1EST01027, Vitamin D3 up-regulated protein 1

Entrez Gene IDs

10628 (Human); 56338 (Mouse); 117514 (Rat)

Gene Symbol

TXNIP

UniProt

Q9H3M7 (Human)

Additional TXNIP Products

Product Documents for TXNIP Antibody (JY2) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for TXNIP Antibody (JY2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

TXNIP Antibody (JY2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54578

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all TXNIP Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for TXNIP Antibody (JY2) - Azide and BSA Free

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] -

Applications for TXNIP Antibody (JY2) - Azide and BSA Free

Flow Cytometry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Knockout Validated

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: TXNIP

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional TXNIP Products

Product Documents for TXNIP Antibody (JY2) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for TXNIP Antibody (JY2) - Azide and BSA Free