TYW3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88492

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88492
NBP1-88492-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TYW3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EFRKWKAQCLSKADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVQKQNCCWLLVTHKLCVKDDVIV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TYW3 Antibody

Western Blot: TYW3 Antibody [NBP1-88492]

Western Blot: TYW3 Antibody [NBP1-88492]

Western Blot: TYW3 Antibody [NBP1-88492] - Analysis in control (vector only transfected HEK293T lysate) and TYW3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TYW3 Antibody [NBP1-88492]

Immunocytochemistry/ Immunofluorescence: TYW3 Antibody [NBP1-88492]

Immunocytochemistry/Immunofluorescence: TYW3 Antibody [NBP1-88492] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: TYW3 Antibody [NBP1-88492]

Immunohistochemistry-Paraffin: TYW3 Antibody [NBP1-88492]

Immunohistochemistry-Paraffin: TYW3 Antibody [NBP1-88492] - Staining of human testis shows moderate cytoplasmic positivity in seminiferus duct cells.

Applications for TYW3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TYW3

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM]

Alternate Names

C1orf171, chromosome 1 open reading frame 171, EC 2.1.1, EC 2.1.1.-, FLJ40918, tRNA wybutosine-synthesizing protein 3 homolog, tRNA-yW synthesizing protein 3 homolog (S. cerevisiae), tRNA-yW-synthesizing protein 3

Gene Symbol

TYW3

Additional TYW3 Products

Product Documents for TYW3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TYW3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.