U11/U12-35K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20774

Novus Biologicals, part of Bio-Techne
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NBP2-20774
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all U11/U12-35K Antibodies »

Product Summary for U11/U12-35K Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human U11/U12-35K. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for U11/U12-35K Antibody

Western Blot: U11/U12-35K Antibody [NBP2-20774]

Western Blot: U11/U12-35K Antibody [NBP2-20774]

Western Blot: U11/U12-35K Antibody [NBP2-20774] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: U11/U12-35K Antibody [NBP2-20774]

Immunocytochemistry/ Immunofluorescence: U11/U12-35K Antibody [NBP2-20774]

Immunocytochemistry/Immunofluorescence: U11/U12-35K Antibody [NBP2-20774] - U11/U12-35K antibody detects U11/U12-35K protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: U11/U12-35K protein stained by U11/U12-35K antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: U11/U12-35K Antibody [NBP2-20774]

Immunohistochemistry-Paraffin: U11/U12-35K Antibody [NBP2-20774]

Immunohistochemistry-Paraffin: U11/U12-35K Antibody [NBP2-20774] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
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Applications for U11/U12-35K Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: U11/U12-35K

The protein encoded by this gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Alternate Names

HM1, HM-1, MGC138160, Protein HM-1, small nuclear ribonucleoprotein 35kDa (U11/U12), U1 snRNP-binding protein homolog, U11/U12 small nuclear ribonucleoprotein 35 kDa protein, U11/U12 snRNP 35 kDa protein, U11/U12 snRNP 35K, U11/U12-35K, U1-snRNP binding protein homolog, U1SNRNPBPU1 snRNP binding protein homolog

Gene Symbol

SNRNP35

UniProt

Q16560 (Human)

Additional U11/U12-35K Products

Product Documents for U11/U12-35K Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for U11/U12-35K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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