U1A Antibody (8I3P5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16247

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16247-100ul
NBP3-16247-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8I3P5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all U1A Antibodies »

Product Summary for U1A Antibody (8I3P5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U1A (P09012). MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U1A Antibody (8I3P5)

Western Blot: U1A Antibody (8I3P5) [NBP3-16247]

Western Blot: U1A Antibody (8I3P5) [NBP3-16247]

Western Blot: U1A Antibody (8I3P5) [NBP3-16247] - Western blot analysis of extracts of various cell lines, using U1A Rabbit mAb (NBP3-16247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: U1A Antibody (8I3P5) [NBP3-16247]

Immunocytochemistry/ Immunofluorescence: U1A Antibody (8I3P5) [NBP3-16247]

Immunocytochemistry/Immunofluorescence: U1A Antibody (8I3P5) [NBP3-16247] - Immunofluorescence analysis of U-2 OS cells using U1A Rabbit mAb (NBP3-16247) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for U1A Antibody (8I3P5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: U1A

U1A binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and p

Alternate Names

Mud1, small nuclear ribonucleoprotein polypeptide A, U1 snRNP A, U1 snRNP-specific protein A, U1-AU1 small nuclear ribonucleoprotein A, U1AU1 small nuclear RNP-specific A

Gene Symbol

SNRPA

Additional U1A Products

Product Documents for U1A Antibody (8I3P5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for U1A Antibody (8I3P5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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