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U1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33465

Novus Biologicals, part of Bio-Techne
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NBP2-33465
NBP2-33465-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for U1A Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U1A Antibody

Western Blot: U1A Antibody [NBP2-33465]

Western Blot: U1A Antibody [NBP2-33465]

Western Blot: U1A Antibody [NBP2-33465] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465]

Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465]

Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465] - Staining of breast cancer.
Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465]

Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465]

Immunohistochemistry-Paraffin: U1A Antibody [NBP2-33465] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Applications for U1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U1A

U1A binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and p

Alternate Names

Mud1, small nuclear ribonucleoprotein polypeptide A, U1 snRNP A, U1 snRNP-specific protein A, U1-AU1 small nuclear ribonucleoprotein A, U1AU1 small nuclear RNP-specific A

Gene Symbol

SNRPA

UniProt

Additional U1A Products

Product Documents for U1A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for U1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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