U2AF2 Antibody (5G8)
Novus Biologicals, part of Bio-Techne | Catalog # H00011338-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5G8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
U2AF2 (NP_001012496.1, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSE
Specificity
This product is specific for Human U2AF2 monoclonal antibody (M03), clone 5G8 [Gene ID: 11338].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for U2AF2 Antibody (5G8)
![Western Blot: U2AF2 Antibody (5G8) [H00011338-M03]](https://resources.bio-techne.com/images/products/U2AF2-Antibody-5G8-Western-Blot-H00011338-M03-img0003.jpg "Western Blot: U2AF2 Antibody (5G8) [H00011338-M03]")
Western Blot: U2AF2 Antibody (5G8) [H00011338-M03]
Western Blot: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of U2AF2 expression in HepG2.![Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03]](https://resources.bio-techne.com/images/products/U2AF2-Antibody-5G8-Immunocytochemistry-Immunofluorescence-H00011338-M03-img0001.jpg "Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03]")
Immunocytochemistry/ Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03]
Immunocytochemistry/Immunofluorescence: U2AF2 Antibody (5G8) [H00011338-M03] - Analysis of monoclonal antibody to U2AF2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: U2AF2 Antibody (5G8) [H00011338-M03] - Detection limit for recombinant GST tagged U2AF2 is 0.3 ng/ml as a capture antibody.
Applications for U2AF2 Antibody (5G8)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a partial recombinant U2AF2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: U2AF2
Alternate Names
hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit
Gene Symbol
U2AF2
UniProt
Additional U2AF2 Products
Product Documents for U2AF2 Antibody (5G8)
Product Specific Notices for U2AF2 Antibody (5G8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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