Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all U2AF2 Antibodies »

Product Summary for U2AF2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 380 and 430 of human U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD) using the numbering given in entry NP_009210.1 (GeneID 11338).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U2AF2 Antibody

Immunohistochemistry: U2AF2 Antibody [NBP2-04139]

Immunohistochemistry: U2AF2 Antibody [NBP2-04139]

Immunohistochemistry: U2AF2 Antibody [NBP2-04139] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: U2AF2 Antibody [NBP2-04139]

Immunoprecipitation: U2AF2 Antibody [NBP2-04139]

Immunoprecipitation: U2AF2 Antibody [NBP2-04139] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04138 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and NBP2-04140, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Immunoprecipitation: U2AF2 Antibody [NBP2-04139]

Immunoprecipitation: U2AF2 Antibody [NBP2-04139]

Immunoprecipitation: U2AF2 Antibody [NBP2-04139] - Detection of human U2AF65 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04139 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and one other rabbit anti-U2AF65 antibody, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for U2AF2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: U2AF2

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Alternate Names

hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit

Gene Symbol

U2AF2

UniProt

NP_009210.1 (Human)

Additional U2AF2 Products

Product Documents for U2AF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for U2AF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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