UAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89197

Novus Biologicals, part of Bio-Techne
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NBP1-89197
NBP1-89197-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UAP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%). Reactivity reported in scientific literature (PMID: 23724116)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UAP1 Antibody

Western Blot: UAP1 Antibody [NBP1-89197]

Western Blot: UAP1 Antibody [NBP1-89197]

Western Blot: UAP1 Antibody [NBP1-89197] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-89197]

Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-89197]

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-89197] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-89197]

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-89197]

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-89197] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
View 2 more images

Applications for UAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UAP1

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P

Alternate Names

AGX, AGX1, AGX2, Antigen X, EC 2.7.7, EC 2.7.7.23, EC 2.7.7.27, SPAG2, sperm associated antigen 2, Sperm-associated antigen 2, UDP-N-acetylhexosamine pyrophosphorylase, UDP-N-acteylglucosamine pyrophosphorylase 1

Gene Symbol

UAP1

Additional UAP1 Products

Product Documents for UAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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