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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Synthetic peptide corresponding to residues near C-terminus of human UBA52 (NP_003324).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBA52 Antibody (OTI4F2) - Azide and BSA Free

Western Blot: UBA52 Antibody (OTI4F2)Azide and BSA Free [NBP2-74730]

Western Blot: UBA52 Antibody (OTI4F2)Azide and BSA Free [NBP2-74730]

Western Blot: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA52.
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730]

Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730]

Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730]

Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730]

Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for UBA52 Antibody (OTI4F2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBA52

UBA52 - ubiquitin A-52 residue ribosomal protein fusion product 1

Alternate Names

CEP52ubiquitin carboxyl extension protein 52, HUBCEP52, MGC126879, MGC126881, MGC57125, RPL40, UBCEP2, ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40, ubiquitin-52 amino acid fusion protein, ubiquitin-60S ribosomal protein L40, ubiquitin-CEP52

Gene Symbol

UBA52

Additional UBA52 Products

Product Documents for UBA52 Antibody (OTI4F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBA52 Antibody (OTI4F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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