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UBAC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81842

Novus Biologicals, part of Bio-Techne
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NBP1-81842
NBP1-81842-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEMGFDEKEVIDALRVNNNQQNAACEWLLGDRKPSPEELDKGIDPDSPLFQAILDNPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBAC1 Antibody

Western Blot: UBAC1 Antibody [NBP1-81842]

Western Blot: UBAC1 Antibody [NBP1-81842]

Western Blot: UBAC1 Antibody [NBP1-81842] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: UBAC1 Antibody [NBP1-81842]

Immunocytochemistry/ Immunofluorescence: UBAC1 Antibody [NBP1-81842]

Immunocytochemistry/Immunofluorescence: UBAC1 Antibody [NBP1-81842] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
Immunohistochemistry-Paraffin: UBAC1 Antibody [NBP1-81842]

Immunohistochemistry-Paraffin: UBAC1 Antibody [NBP1-81842]

Immunohistochemistry-Paraffin: UBAC1 Antibody [NBP1-81842] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for UBAC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBAC1

Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle

Alternate Names

E3 ubiquitin-protein ligase subunit KPC2, GBDR1UBA domain-containing protein 1, Glialblastoma cell differentiation-related protein 1, Kip1 ubiquitination-promoting complex protein 2, KPC2, putative glialblastoma cell differentiation-related protein, RP11-432J22.3, UBA domain containing 1, UBADC1, ubiquitin associated domain containing 1, ubiquitin-associated domain-containing protein 1

Gene Symbol

UBAC1

Additional UBAC1 Products

Product Documents for UBAC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBAC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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