UbcH5a/UBE2D1 Antibody (2C6)

Novus Biologicals, part of Bio-Techne | Catalog # H00007321-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH5a/UBE2D1 Antibodies »

Product Specifications

Immunogen

UBE2D1 (NP_003329, 1 a.a. ~ 94 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWS

Specificity

UBE2D1 (2C6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UbcH5a/UBE2D1 Antibody (2C6)

Western Blot: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01]

Western Blot: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Analysis of UBE2D1 expression in transfected 293T cell line by UBE2D1 monoclonal antibody (M01), clone 2C6.Lane 1: UBE2D1 transfected lysate(16.6 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Analysis of monoclonal antibody to UBE2D1 on formalin-fixed paraffin-embedded human cervix cancer. Antibody concentration 1 ug/ml.

Sandwich ELISA: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Detection limit for recombinant GST tagged UBE2D1 is approximately 0.3ng/ml as a capture antibody.

Applications for UbcH5a/UBE2D1 Antibody (2C6)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH5a/UBE2D1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Alternate Names

E2(17)KB1, UBC4/5, UBC5A, UBE2D1

Entrez Gene IDs

7321 (Human)

Gene Symbol

UBE2D1

OMIM

602961 (Human)

UniProt

NP_003329 (Human)

Additional UbcH5a/UBE2D1 Products

Product Documents for UbcH5a/UBE2D1 Antibody (2C6)

Product Datasheets

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COA

SDS

Product Specific Notices for UbcH5a/UBE2D1 Antibody (2C6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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