Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C2

Concentration

1 mg/ml

View all UbcH5b/UBE2D2 Antibodies »

Product Summary for UbcH5b/UBE2D2 Antibody (OTI2C2)

Immunogen

Full length human recombinant protein of human UBE2D2 (NP_003330) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH5b/UBE2D2 Antibody (OTI2C2)

Western Blot: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Western Blot: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Western Blot: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2.
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270]

Immunohistochemistry: UbcH5b/UBE2D2 Antibody (OTI2C2) [NBP2-46270] - UbcH5b/UBE2D2 Antibody (2C2) [NBP2-46270] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)
View 21 more images

Applications for UbcH5b/UBE2D2 Antibody (OTI2C2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH5b/UBE2D2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

PUBC1, UBC5B, UBE2D2

Gene Symbol

UBE2D2

Additional UbcH5b/UBE2D2 Products

Product Documents for UbcH5b/UBE2D2 Antibody (OTI2C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UbcH5b/UBE2D2 Antibody (OTI2C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.