Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all UbcH7/UBE2L3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and the C-terminus (residue 154) of human Ubiquitin-Conjugating Enzyme H7 using the numbering given in entry NP_003338.1 (GeneID 7332).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265]

Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265]

Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265] - Detection of Human and Mouse UBCH7 one HeLa whole cell lysate using NB100-2265.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti- UBCH7 antibody used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - Human breast tumors stained with UbcH7 antibody. IHC-P image submitted by a verified customer review.

Applications for UbcH7/UBE2L3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 5 ug / mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
This UbcH7/UBE2L3 antibody is validated for IHC-P from a verified customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation: efficiency in IP is low.

Reviewed Applications

Read 1 review rated 5 using NB100-2265 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Entrez Gene IDs

7332 (Human); 22195 (Mouse)

Gene Symbol

UBE2L3

UniProt

P68036 (Human)

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UbcH7/UBE2L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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