UbcH7/UBE2L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-841

Novus Biologicals, part of Bio-Techne
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NB300-841
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all UbcH7/UBE2L3 Antibodies »

Product Summary for UbcH7/UBE2L3 Antibody

Immunogen

Peptide with sequence C-EFTKKYGEKRPVD corresponding to C-Terminus according to NP_003338.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer).
Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of Hela lysate (RIPA buffer, 35ug total protein per lane).
Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841]

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer).
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Applications for UbcH7/UBE2L3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 18 kDa band observed in HeLa, HEK293, HepG2, and Jurkat lysates, in human, mouse, and rat heart, skeletal muscle, and testis lysates, and in porcine heart lysate (calculated MW of 17.9 kDa band according to human NP_003338.1 and mouse NP_033482.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Ube2L3 gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Ubiquitous, with highest expression in testis.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Entrez Gene IDs

7332 (Human); 22195 (Mouse)

Gene Symbol

UBE2L3

UniProt

NP_005330. (Human)

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH7/UBE2L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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