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UbcH7/UBE2L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20786

Novus Biologicals, part of Bio-Techne
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NBP2-20786

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UbcH7/UBE2L3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH7/UBE2L3 Antibody

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-20786]

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-20786]

Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786]

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786]

Western Blot: Ube2L3 Antibody [NBP2-20786] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 12% SDS PAGE gel, diluted at 1:1000.
UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] -

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.

Applications for UbcH7/UBE2L3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-20786 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

UniProt

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UbcH7/UBE2L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.