UbcH7/UBE2L3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49367

Novus Biologicals, part of Bio-Techne
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NBP2-49367
NBP2-49367-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH7/UBE2L3 Antibodies »

Product Summary for UbcH7/UBE2L3 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: CLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-49367]

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-49367]

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-49367] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-49367]

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-49367]

Immunocytochemistry/Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-49367] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP2-49367]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP2-49367]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP2-49367] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.

Applications for UbcH7/UBE2L3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UbcH7/UBE2L3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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