UbcH8/Ube2L6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86885

Novus Biologicals, part of Bio-Techne
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NBP1-86885
NBP1-86885-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH8/Ube2L6 Antibody

Western Blot: UbcH8/Ube2L6 Antibody [NBP1-86885]

Western Blot: UbcH8/Ube2L6 Antibody [NBP1-86885]

Western Blot: UbcH8/Ube2L6 Antibody [NBP1-86885] - Analysis in control (vector only transfected HEK293T lysate) and UBE2L6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: UbcH8/Ube2L6 Antibody [NBP1-86885]

Immunocytochemistry/ Immunofluorescence: UbcH8/Ube2L6 Antibody [NBP1-86885]

Immunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody [NBP1-86885] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody [NBP1-86885]

Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody [NBP1-86885]

Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody [NBP1-86885] - Staining of human spleen shows cytoplasmic positivity in cells in white pulp.

Applications for UbcH8/Ube2L6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH8

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Alternate Names

RIG-B, UBE2L6

Gene Symbol

UBE2L6

Additional UbcH8 Products

Product Documents for UbcH8/Ube2L6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH8/Ube2L6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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