UBE2E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55077

Novus Biologicals, part of Bio-Techne
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NBP1-55077
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all UBE2E2 Antibodies »

Product Summary for UBE2E2 Antibody

Immunogen

Synthetic peptides corresponding to UBE2E2(ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)) The peptide sequence was selected from the N terminal of UBE2E2. Peptide sequence GDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLD. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for UBE2E2 Antibody

Western Blot: UBE2E2 Antibody [NBP1-55077]

Western Blot: UBE2E2 Antibody [NBP1-55077]

Western Blot: UBE2E2 Antibody [NBP1-55077] - Lanes: 1 : 40 ng HIS-UBE2D1 protein2: 40 ng HIS-UBE2D2 protein3: 40 ng HIS-UBE2D3 protein4: 40 ng HIS-UBE2D4 protein5: 40 ng HIS-UBE2E1 protein6: 40 ng HIS-UBE2E2 protein7: 40 ng HIS-UBE2E3 protein8: 40 ng HIS-UBE2K protein9: 40 ng HIS-UBE2L3 protein10: 40 ng HIS-UBE2N protein11 : 40 ng HIS-UBE2V1 protein12: 40 ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 50,000 Gene name: UBE2E2 Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-55077]

Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-55077]

Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-55077] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: UBE2E2 Antibody [NBP1-55077]

Western Blot: UBE2E2 Antibody [NBP1-55077]

Western Blot: UBE2E2 Antibody [NBP1-55077] - Jurkat cell lysate, Antibody Titration: 0.125ug/ml

Applications for UBE2E2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2E2

UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.

Alternate Names

EC 6.3.2.19, FLJ25157, UbcH8, Ubiquitin carrier protein E2, ubiquitin-conjugating enzyme E2 E2, ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5), ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase E2

Gene Symbol

UBE2E2

UniProt

Q96LR5 (Human)

Additional UBE2E2 Products

Product Documents for UBE2E2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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