UBE2E3 Antibody (3E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00010477-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2E3 Antibodies »

Product Specifications

Immunogen

UBE2E3 (NP_006348, 65 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDN

Specificity

UBE2E3 - ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) (3E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UBE2E3 Antibody (3E9)

Immunocytochemistry/ Immunofluorescence: UBE2E3 Antibody (3E9) [H00010477-M05]

Immunocytochemistry/ Immunofluorescence: UBE2E3 Antibody (3E9) [H00010477-M05]

Immunocytochemistry/Immunofluorescence: UBE2E3 Antibody (3E9) [H00010477-M05] - Analysis of monoclonal antibody to UBE2E3 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: UBE2E3 Antibody (3E9) [H00010477-M05]

ELISA: UBE2E3 Antibody (3E9) [H00010477-M05]

ELISA: UBE2E3 Antibody (3E9) [H00010477-M05] - Detection limit for recombinant GST tagged UBE2E3 is approximately 3ng/ml as a capture antibody.

Applications for UBE2E3 Antibody (3E9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2E3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Entrez Gene IDs

10477 (Human)

Gene Symbol

UBE2E3

UniProt

NP_006348 (Human)

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (3E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE2E3 Antibody (3E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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