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UBE2E3 Antibody (OTI1B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46271

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B4

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2E3 Antibody (OTI1B4)

Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Adenocarcinoma of Human endometrium tissue.
Flow Cytometry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Flow Cytometry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Flow Cytometry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by UBE2E3 antibody.

Applications for UBE2E3 Antibody (OTI1B4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2E3

Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Gene Symbol

UBE2E3

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (OTI1B4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2E3 Antibody (OTI1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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