UBE2E3 Antibody (OTI7E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03819

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7E8

Concentration

1 mg/ml

View all UBE2E3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2E3 Antibody (OTI7E8)

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody, and then analyzed by flow cytometry.
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Applications for UBE2E3 Antibody (OTI7E8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 1 review rated 5 using NBP2-03819 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2E3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Entrez Gene IDs

10477 (Human)

Gene Symbol

UBE2E3

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (OTI7E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2E3 Antibody (OTI7E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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