UBE2F/NCE2 Antibody (SR2446)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27737

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-27737
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2446

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2F Antibodies »

Product Summary for UBE2F/NCE2 Antibody (SR2446)

Immunogen

Recombinant protein of human UBE2F/NCE2 (Uniprot #: Q969M7)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2F/NCE2 Antibody (SR2446)

UBE2F/NCE2 Antibody (SR2446)

Western Blot: UBE2F/NCE2 Antibody (SR2446) [NBP3-27737] -

Western Blot: UBE2F/NCE2 Antibody (SR2446) [NBP3-27737] - Western blot analysis of Hela, MCF-7 lysates

Applications for UBE2F/NCE2 Antibody (SR2446)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2F

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Long Name

Ubiquitin-conjugating Enzyme E2F

Alternate Names

NCE2

Gene Symbol

UBE2F

Additional UBE2F Products

Product Documents for UBE2F/NCE2 Antibody (SR2446)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2F/NCE2 Antibody (SR2446)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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