UBE2I/Ubc9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00096

Novus Biologicals, part of Bio-Techne
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NBP3-00096-100ul
NBP3-00096-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2I/Ubc9 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human UBE2I/Ubc9 (Accession#: P63279; Met1-Ser158)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBE2I/Ubc9 Antibody

Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096]

Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096]

Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - Anti-UBE2I/Ubc9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa
Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096]

Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096]

Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human UBE2I/Ubc9 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096]

Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096]

Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I/Ubc9 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate Lane B: 0.5 mg Hela Whole Cell Lysate Lane C: 0.5 mg A431 Whole Cell Lysate 2 ul anti-UBE2I/Ubc9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UBE2I/Ubc9 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa

Applications for UBE2I/Ubc9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2I/Ubc9

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Gene Symbol

UBE2I

Additional UBE2I/Ubc9 Products

Product Documents for UBE2I/Ubc9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UBE2I/Ubc9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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