UBE2J2/UBC6 Antibody

Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] -

Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] - TMEM129 & Ube2J2 are required for US11-induced FcRn ubiquitination, a, b TMEM129 & Ube2J2 is required for US11-induced FcRn ubiquitination. HeLaFcRn+US11 cells were transfected with 20 nM TMEM129, Ube2J1, or Ube2J2 siRNA oligomers for 48 h or empty vector. Cells were subsequently treated with 50 μM MG132 for 4 h & then lysed. All immunoprecipitates or cell lysates (20 μg) as internal controls were subject to western blotting with corresponding Abs as indicated. c, d HeLaUS11+FcRn cells were transfected with 20 nM Ube2J1 (c, top) & Ube2J2 (c, bottom) siRNA oligomers for 48 h. Cells were then treated with CHX (100 μg/ml) & chased for the indicated time. The cells were lysed after CHX treatment & cell lysates (20 μg) were subject to western blotting with Abs as indicated. The level of remaining FcRn (d) in Ube2j1 siRNA-treated cells (black) or Ube2j2 siRNA-treated cells (red) was quantified as the percentage of the beta-tubulin level at different time points. The percentage of time point 0 (min) is assigned a value of 100% & the values from other time points are normalized to this value. Error bars represented mean ± SEM of three independent repeats Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31289263), licensed under a CC-BY license. Not internally tested by Novus Biologicals.