UBE2J2/UBC6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78071
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified UBE2J2/UBC6 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human UBE2J2/UBC6 protein. (Uniprot: Q8N2K1)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with UBE2J2/UBC6 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Localization
Endoplasmic reticulum
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against human UBE2J2/UBC6 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for UBE2J2/UBC6 Antibody
Western Blot: UBE2J2/UBC6 Antibody [NBP1-78071]
Western Blot: UBE2J2/UBC6 Antibody [NBP1-78071] - expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.
N/A: UBE2J2/UBC6 Antibody [NBP1-78071]
UBE2J2/UBC6 Antibody
Western blot using affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over-expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MDApplications for UBE2J2/UBC6 Antibody
Application
Recommended Usage
ELISA
1:5000-1:25000
Western Blot
1:500-1:3000
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: UBE2J2
Long Name
Ubiquitin-conjugating Enzyme E2, J2
Alternate Names
NCUBE2, PRO2121, Ubc6, Ubc6p
Gene Symbol
UBE2J2
UniProt
Additional UBE2J2 Products
Product Documents for UBE2J2/UBC6 Antibody
Product Specific Notices for UBE2J2/UBC6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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