Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UBE2M/Ubc12 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2M(NP_003960) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free

Western Blot: UBE2M/Ubc12 Antibody (OTI2D9)Azide and BSA Free [NBP2-74748]

Western Blot: UBE2M/Ubc12 Antibody (OTI2D9)Azide and BSA Free [NBP2-74748]

Western Blot: UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free [NBP2-74748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M.
Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free [NBP2-74748]

Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free [NBP2-74748]

Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free [NBP2-74748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
Western Blot: UBE2M/Ubc12 Antibody (OTI2D9)Azide and BSA Free [NBP2-74748]

Western Blot: UBE2M/Ubc12 Antibody (OTI2D9)Azide and BSA Free [NBP2-74748]

Western Blot: UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free [NBP2-74748] - Analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.

Applications for UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2M/Ubc12

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Alternate Names

UBC-RS2, UBC12, UbcH12

Gene Symbol

UBE2M

Additional UBE2M/Ubc12 Products

Product Documents for UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2M/Ubc12 Antibody (OTI2D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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