UBE2M/Ubc12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00093

Novus Biologicals, part of Bio-Techne
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NBP3-00093-100ul
NBP3-00093-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2M/Ubc12 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human UBE2M/Ubc12 (Accession#: P61081; Met1-Lys183)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBE2M/Ubc12 Antibody

Western Blot: UBE2M/Ubc12 Antibody [NBP3-00093]

Western Blot: UBE2M/Ubc12 Antibody [NBP3-00093]

Western Blot: UBE2M/Ubc12 Antibody [NBP3-00093] - Anti-UBE2M/Ubc12 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: UBE2M/Ubc12 Antibody [NBP3-00093]

Immunoprecipitation: UBE2M/Ubc12 Antibody [NBP3-00093]

Immunoprecipitation: UBE2M/Ubc12 Antibody [NBP3-00093] - UBE2M/Ubc12 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-UBE2M/Ubc12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-UBE2M/Ubc12 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa

Applications for UBE2M/Ubc12 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2M/Ubc12

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Alternate Names

UBC-RS2, UBC12, UbcH12

Gene Symbol

UBE2M

Additional UBE2M/Ubc12 Products

Product Documents for UBE2M/Ubc12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2M/Ubc12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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