UBE2R1/CDC34 Antibody (14B5)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-168

Novus Biologicals, part of Bio-Techne
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NB500-168
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 14B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2R1/CDC34 Antibodies »

Product Summary for UBE2R1/CDC34 Antibody (14B5)

Immunogen

Full-length human UBE2R1/CDC34 expressed in E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2R1/CDC34 Antibody (14B5)

Western Blot: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Western Blot: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Western Blot: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Analysis of paraformaldehyde-fixed HeLa, using CDC34 antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (14B5) [NB500-168]

Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Analysis of paraffin-embedded HBL435 xenograft, using CDC34 antibody at 1:200 dilution.

Applications for UBE2R1/CDC34 Antibody (14B5)

Application
Recommended Usage

Immunohistochemistry

10 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2R1/CDC34

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Alternate Names

Cdc34, UBC3

Entrez Gene IDs

997 (Human)

Gene Symbol

CDC34

Additional UBE2R1/CDC34 Products

Product Documents for UBE2R1/CDC34 Antibody (14B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2R1/CDC34 Antibody (14B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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