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UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07514

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-CDC34-2

Concentration

0.2 mg/ml

Product Summary for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Immunogen

Recombinant human full-length UBE2R1/CDC34 protein (Uniprot: P49427)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08839)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-UBE2R1/CDC34-2).
Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-UBE2R1/CDC34-2).
Protein Array: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Protein Array: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514]

Protein Array: UBE2R1/CDC34 Antibody (CPTC-CDC34-2) [NBP3-07514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using UBE2R1/CDC34 Mouse Monoclonal Antibody (CPTC-CDC34-2).

Applications for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UBE2R1/CDC34

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Alternate Names

Cdc34, UBC3

Gene Symbol

CDC34

UniProt

Additional UBE2R1/CDC34 Products

Product Documents for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2R1/CDC34 Antibody (CPTC-CDC34-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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