UBE2R1/CDC34 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32153
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (97%), Rat (98%), Xenopus (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2R1/CDC34. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (90%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UBE2R1/CDC34 Antibody
![Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]](https://resources.bio-techne.com/images/products/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0007.jpg "Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]")
Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]
Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.![Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]](https://resources.bio-techne.com/images/products/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0004.jpg "Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]")
Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]
Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for UBE2R1/CDC34 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: UBE2R1/CDC34
Alternate Names
Cdc34, UBC3
Gene Symbol
CDC34
Additional UBE2R1/CDC34 Products
Product Documents for UBE2R1/CDC34 Antibody
Product Specific Notices for UBE2R1/CDC34 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.