UBE2R1/CDC34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84946

Novus Biologicals, part of Bio-Techne
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NBP1-84946
NBP1-84946-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2R1/CDC34 Antibodies »

Product Summary for UBE2R1/CDC34 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2R1/CDC34 Antibody

Western Blot: UBE2R1/CDC34 Antibody [NBP1-84946]

Western Blot: UBE2R1/CDC34 Antibody [NBP1-84946]

Western Blot: UbcH3/Cdc34 Antibody [NBP1-84946] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-112
Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-84946]

Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-84946]

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-84946] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.
Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP1-84946]

Immunohistochemistry-Paraffin: UBE2R1/CDC34 Antibody [NBP1-84946]

Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody [NBP1-84946] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.

Applications for UBE2R1/CDC34 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2R1/CDC34

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Alternate Names

Cdc34, UBC3

Gene Symbol

CDC34

Additional UBE2R1/CDC34 Products

Product Documents for UBE2R1/CDC34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2R1/CDC34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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