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UBE2S Antibody (2C12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41126

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C12

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human UBE2S (1-222aa) purified from E. coli (NP_055316.2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2S Antibody (2C12) - BSA Free

Western Blot: UBE2S Antibody (2C12)BSA Free [NBP1-41126]

Western Blot: UBE2S Antibody (2C12)BSA Free [NBP1-41126]

Western Blot: UBE2S Antibody (2C12) [NBP1-41126] - Cell lysates of 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: UBE2S Antibody (2C12) - BSA Free [NBP1-41126]

Immunocytochemistry/ Immunofluorescence: UBE2S Antibody (2C12) - BSA Free [NBP1-41126]

Immunocytochemistry/Immunofluorescence: UBE2S Antibody (2C12) [NBP1-41126] - Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).

Applications for UBE2S Antibody (2C12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:250-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2S

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Long Name

Ubiquitin-conjugating Enzyme E2S

Alternate Names

E2-24K, E2-EPF, E2-EPF52, EPF5

Entrez Gene IDs

27338 (Human)

Gene Symbol

UBE2S

UniProt

Additional UBE2S Products

Product Documents for UBE2S Antibody (2C12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2S Antibody (2C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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