UBE2T Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1150

Novus Biologicals, part of Bio-Techne
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NB100-1150
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all UBE2T Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QLVGIEKKFHPDV corresponding to C-Terminus according to NP_054895.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBE2T Antibody

Western Blot: UBE2T Antibody [NB100-1150]

Western Blot: UBE2T Antibody [NB100-1150]

Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for UBE2T Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in lysates of K562, HEK293 and A549 (calculated MW of 22.5 kDa band according to NP_054895.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2T

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

Long Name

Ubiquitin-conjugating Enzyme E2T

Alternate Names

HSPC150, PIG50

Entrez Gene IDs

29089 (Human)

Gene Symbol

UBE2T

UniProt

NP_054895. (Human)

Additional UBE2T Products

Product Documents for UBE2T Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2T Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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