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UBE3C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81558

Novus Biologicals, part of Bio-Techne
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NBP1-81558
NBP1-81558-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for UBE3C Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE3C Antibody

Immunocytochemistry/ Immunofluorescence: UBE3C Antibody [NBP1-81558]

Immunocytochemistry/ Immunofluorescence: UBE3C Antibody [NBP1-81558]

Immunocytochemistry/Immunofluorescence: UBE3C Antibody [NBP1-81558] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: UBE3C Antibody [NBP1-81558]

Immunohistochemistry-Paraffin: UBE3C Antibody [NBP1-81558]

Immunohistochemistry-Paraffin: UBE3C Antibody [NBP1-81558] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for UBE3C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE3C

E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo

Alternate Names

EC 6.3.2.-, KIAA0010KIAA10ubiquitin-protein isopeptide ligase (E3), ubiquitin protein ligase E3C, ubiquitin-protein ligase E3C

Gene Symbol

UBE3C

Additional UBE3C Products

Product Documents for UBE3C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE3C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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