UBE4A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56653

Novus Biologicals, part of Bio-Techne
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NBP2-56653
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE4A Antibodies »

Product Summary for UBE4A Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE4A Antibody

Western Blot: UBE4A Antibody [NBP2-56653]

Western Blot: UBE4A Antibody [NBP2-56653]

Western Blot: UBE4A Antibody [NBP2-56653] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: UBE4A Antibody [NBP2-56653]

Immunocytochemistry/ Immunofluorescence: UBE4A Antibody [NBP2-56653]

Immunocytochemistry/Immunofluorescence: UBE4A Antibody [NBP2-56653] - Staining of human cell line MCF7 shows localization to nuclear speckles & nucleoli fibrillar center.
Western Blot: UBE4A Antibody [NBP2-56653]

Western Blot: UBE4A Antibody [NBP2-56653]

Western Blot: UBE4A Antibody [NBP2-56653] - Analysis in human cell line HEL.

Applications for UBE4A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE4A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Alternate Names

E4MGC133315, KIAA0126ubiquitination factor E4A (homologous to yeast UFD2), ubiquitination factor E4A (UFD2 homolog, yeast), UBOX2, UFD2ubiquitin conjugation factor E4 A

Gene Symbol

UBE4A

Additional UBE4A Products

Product Documents for UBE4A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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