UBE4A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98836

Novus Biologicals, part of Bio-Techne
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NBP2-98836-100ul
NBP2-98836-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE4A Antibodies »

Product Summary for UBE4A Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human UBE4A.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBE4A Antibody

Western Blot: UBE4A Antibody [NBP2-98836]

Western Blot: UBE4A Antibody [NBP2-98836]

Western Blot: UBE4A Antibody [NBP2-98836] - Lane A: 293T Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:123 kDaObserved band size:125 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: UBE4A Antibody [NBP2-98836]

Immunoprecipitation: UBE4A Antibody [NBP2-98836]

Immunoprecipitation: UBE4A Antibody [NBP2-98836] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-UBE4A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UBE4A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 123 kDaObserved band size: 130 kDa

Applications for UBE4A Antibody

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE4A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Alternate Names

E4MGC133315, KIAA0126ubiquitination factor E4A (homologous to yeast UFD2), ubiquitination factor E4A (UFD2 homolog, yeast), UBOX2, UFD2ubiquitin conjugation factor E4 A

Gene Symbol

UBE4A

Additional UBE4A Products

Product Documents for UBE4A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for UBE4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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