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UBE4B Antibody (S04-6C6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19924

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19924-100ul
NBP3-19924-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-6C6

Concentration

0.3 mg/ml

Product Summary for UBE4B Antibody (S04-6C6)

Immunogen

A synthetic peptide of human UBE4B (Uniprot # O95155)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE4B Antibody (S04-6C6)

Western Blot: UBE4B Antibody (S04-6C6) [NBP3-19924]

Western Blot: UBE4B Antibody (S04-6C6) [NBP3-19924]

Western Blot: UBE4B Antibody (S04-6C6) [NBP3-19924] - Western blot detection of UBE4B in Hela, A549, HL-60, U2OS, U897-MG cell lysates using NBP3-19924 (1:1000 diluted). Predicted band size: 146kDa. Observed band size: 146kDa.
Immunocytochemistry/ Immunofluorescence: UBE4B Antibody (S04-6C6) [NBP3-19924]

Immunocytochemistry/ Immunofluorescence: UBE4B Antibody (S04-6C6) [NBP3-19924]

Immunocytochemistry/Immunofluorescence: UBE4B Antibody (S04-6C6) [NBP3-19924] - Immunocytochemistry of UBE4B (green) in MCF-7 using NBP3-19924 at dilution 1/20, and DAPI (blue)

Applications for UBE4B Antibody (S04-6C6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE4B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes.

Long Name

Ubiquitination Factor E4B

Alternate Names

UBOX3, UFD2, UFD2a

Gene Symbol

UBE4B

Additional UBE4B Products

Product Documents for UBE4B Antibody (S04-6C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE4B Antibody (S04-6C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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