Ubiquilin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85639

Novus Biologicals, part of Bio-Techne
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NBP1-85639
NBP1-85639-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ubiquilin 2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%). Mouse reactivity reported in scientific literature (PMID: 26141599), (PMID: 26152284).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ubiquilin 2 Antibody

Western Blot: Ubiquilin 2 Antibody [NBP1-85639]

Western Blot: Ubiquilin 2 Antibody [NBP1-85639]

Western Blot: Ubiquilin 2 Antibody [NBP1-85639] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-UBQLN2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639]

Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639]

Immunocytochemistry/Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebellum shows moderate to strong cytoplasmic and nuclear positivity in Purkinje cells.
View 5 more images

Applications for Ubiquilin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in the literature (PMID:26152284). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquilin 2

Ubiquilin 2 encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

Alternate Names

CHAP1, CHAP1/DSK2, DSK2, DSK2 homolog, FLJ10167, FLJ56541, hPLIC-2, N4BP4LIC-2, Nedd4 binding protein 4, PLIC-2, PLIC2Dsk2, Protein linking IAP with cytoskeleton 2, RIHFB2157, ubiquilin 2, ubiquilin-2, Ubiquitin-like product Chap1/Dsk2

Gene Symbol

UBQLN2

Additional Ubiquilin 2 Products

Product Documents for Ubiquilin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquilin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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