Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6C11-B3

Concentration

1 mg/ml

View all Ubiquitin+1 Antibodies »

Product Summary for Ubiquitin B Antibody (6C11-B3)

Immunogen

Native bovine ubiquitin, conjugated to KLH

Localization

Cytoplasm , Nucleus

Specificity

Detects approx 10kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Ubiquitin B Antibody (6C11-B3)

Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Immunocytochemistry/ Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.
Immunocytochemistry/ Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Immunocytochemistry/ Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882]

Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.
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Applications for Ubiquitin B Antibody (6C11-B3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Ubiquitin Antibody was sufficient for detection of ubiquitin in 10 ug of Hela Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquitin+1

1 ug/mL was sufficient for detection of ubiquitin in 10ug of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Gene Symbol

UBB

Additional Ubiquitin+1 Products

Product Documents for Ubiquitin B Antibody (6C11-B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin B Antibody (6C11-B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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