Ubiquitin B Antibody (UBB/1748)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07163

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # UBB/1748

Concentration

0.2 mg/ml

View all Ubiquitin+1 Antibodies »

Product Summary for Ubiquitin B Antibody (UBB/1748)

Immunogen

Recombinant fragment of human Ubiquitin B protein (around aa 1-119) (exact sequence is proprietary)

Localization

Cell Surface, Cytoplasmic and Nuclear

Marker

Autophagy Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08905)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ubiquitin B Antibody (UBB/1748)

Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Western Blot Analysis of human Raji cell lysate using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-07163]

Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Ubiquitin B Antibody (UBB/1748)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ubiquitin+1

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Long Name

frame shift mutant

Alternate Names

FLJ25987, MGC8385, polyubiquitin B, polyubiquitin-B, RPS27A, UBA52, UBC, ubiquitin B

Gene Symbol

UBB

UniProt

P62987 (Human), P62979 (Human), P62988 (Human)

Additional Ubiquitin+1 Products

Product Documents for Ubiquitin B Antibody (UBB/1748)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin B Antibody (UBB/1748)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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